Software:Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Stanley Adoro Apr 10, 2025 QuantStudio six Flex real-time PCR system for RT-qPCR and ChIP-qPCR BioRad iMark Microplate reader and SpectraMax ID3 for reading absorbance for BCA and MTT assays BD LSR Fortessa and BD LSR-II for flow cytometry acquisition Konica Minolta SRX-101a for western blot film development Analytik Jena UVP ChemStudio and BioRad ChemiDoc MP for western blot digital imaging XF96 Extracellular Flux Analyzer (Agilent Technologies) for Seahorse assays Olympus IX73 microscope for histological images Illumina HiSeq X Ten for RNA sequencing (CUTLL1) Illumina NovaSeq 6000 for RNA sequencing (murine) and ChiP sequencing Nikon Eclipse Ti2 microscope (IF images) GraphPad Prism for data analysis and statistical tests FlowJo for flow cytometry analysis ImageJ for western blot quantification and IF analysis Star aligner (v2.7.9a) for RNA sequencing alignment htseq (v0.11.4) to generate gene counts for RNAseq DEseq2 (v1.38.3) for differential expression analysis GSEA 4.3.2 and MsigDB Hallmark gene set for pathway analysis BioMart R and Ensembl release 105 for comparing human and mouse DEGs 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Article Title: Taurine from tumour niche drives glycolysis to promote leukaemogenesis.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Flow cytometry-based analysis was performed on LSR Fortessa and cell sorting was carried out on BD FACS Aria II using FACSDiva v9.0 (BD Biosciences). qRT-PCR was carried out on BioRad CFX96 C100 Thermocycler using BioRad CFX Manager 1.1 v4.1 (BioRad) and Thermofisher Scientific Quant Studio 12K Flex Real Time PCR using using Quant Studio v1.2 (Thermofisher Scientific). .. RNA and DNA concentrations were determined using the NanoDrop 1000 Spectrophotometer (Thermofisher Scientific).
Real-time Polymerase Chain Reaction:Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Stanley Adoro Apr 10, 2025 QuantStudio six Flex real-time PCR system for RT-qPCR and ChIP-qPCR BioRad iMark Microplate reader and SpectraMax ID3 for reading absorbance for BCA and MTT assays BD LSR Fortessa and BD LSR-II for flow cytometry acquisition Konica Minolta SRX-101a for western blot film development Analytik Jena UVP ChemStudio and BioRad ChemiDoc MP for western blot digital imaging XF96 Extracellular Flux Analyzer (Agilent Technologies) for Seahorse assays Olympus IX73 microscope for histological images Illumina HiSeq X Ten for RNA sequencing (CUTLL1) Illumina NovaSeq 6000 for RNA sequencing (murine) and ChiP sequencing Nikon Eclipse Ti2 microscope (IF images) GraphPad Prism for data analysis and statistical tests FlowJo for flow cytometry analysis ImageJ for western blot quantification and IF analysis Star aligner (v2.7.9a) for RNA sequencing alignment htseq (v0.11.4) to generate gene counts for RNAseq DEseq2 (v1.38.3) for differential expression analysis GSEA 4.3.2 and MsigDB Hallmark gene set for pathway analysis BioMart R and Ensembl release 105 for comparing human and mouse DEGs 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Article Title: Taurine from tumour niche drives glycolysis to promote leukaemogenesis.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Flow cytometry-based analysis was performed on LSR Fortessa and cell sorting was carried out on BD FACS Aria II using FACSDiva v9.0 (BD Biosciences). qRT-PCR was carried out on BioRad CFX96 C100 Thermocycler using BioRad CFX Manager 1.1 v4.1 (BioRad) and Thermofisher Scientific Quant Studio 12K Flex Real Time PCR using using Quant Studio v1.2 (Thermofisher Scientific). .. RNA and DNA concentrations were determined using the NanoDrop 1000 Spectrophotometer (Thermofisher Scientific).
Quantitative RT-PCR:Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Stanley Adoro Apr 10, 2025 QuantStudio six Flex real-time PCR system for RT-qPCR and ChIP-qPCR BioRad iMark Microplate reader and SpectraMax ID3 for reading absorbance for BCA and MTT assays BD LSR Fortessa and BD LSR-II for flow cytometry acquisition Konica Minolta SRX-101a for western blot film development Analytik Jena UVP ChemStudio and BioRad ChemiDoc MP for western blot digital imaging XF96 Extracellular Flux Analyzer (Agilent Technologies) for Seahorse assays Olympus IX73 microscope for histological images Illumina HiSeq X Ten for RNA sequencing (CUTLL1) Illumina NovaSeq 6000 for RNA sequencing (murine) and ChiP sequencing Nikon Eclipse Ti2 microscope (IF images) GraphPad Prism for data analysis and statistical tests FlowJo for flow cytometry analysis ImageJ for western blot quantification and IF analysis Star aligner (v2.7.9a) for RNA sequencing alignment htseq (v0.11.4) to generate gene counts for RNAseq DEseq2 (v1.38.3) for differential expression analysis GSEA 4.3.2 and MsigDB Hallmark gene set for pathway analysis BioMart R and Ensembl release 105 for comparing human and mouse DEGs 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Article Title: Taurine from tumour niche drives glycolysis to promote leukaemogenesis.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Flow cytometry-based analysis was performed on LSR Fortessa and cell sorting was carried out on BD FACS Aria II using FACSDiva v9.0 (BD Biosciences). qRT-PCR was carried out on BioRad CFX96 C100 Thermocycler using BioRad CFX Manager 1.1 v4.1 (BioRad) and Thermofisher Scientific Quant Studio 12K Flex Real Time PCR using using Quant Studio v1.2 (Thermofisher Scientific). .. RNA and DNA concentrations were determined using the NanoDrop 1000 Spectrophotometer (Thermofisher Scientific).
ChIP-qPCR:Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Stanley Adoro Apr 10, 2025 QuantStudio six Flex real-time PCR system for RT-qPCR and ChIP-qPCR BioRad iMark Microplate reader and SpectraMax ID3 for reading absorbance for BCA and MTT assays BD LSR Fortessa and BD LSR-II for flow cytometry acquisition Konica Minolta SRX-101a for western blot film development Analytik Jena UVP ChemStudio and BioRad ChemiDoc MP for western blot digital imaging XF96 Extracellular Flux Analyzer (Agilent Technologies) for Seahorse assays Olympus IX73 microscope for histological images Illumina HiSeq X Ten for RNA sequencing (CUTLL1) Illumina NovaSeq 6000 for RNA sequencing (murine) and ChiP sequencing Nikon Eclipse Ti2 microscope (IF images) GraphPad Prism for data analysis and statistical tests FlowJo for flow cytometry analysis ImageJ for western blot quantification and IF analysis Star aligner (v2.7.9a) for RNA sequencing alignment htseq (v0.11.4) to generate gene counts for RNAseq DEseq2 (v1.38.3) for differential expression analysis GSEA 4.3.2 and MsigDB Hallmark gene set for pathway analysis BioMart R and Ensembl release 105 for comparing human and mouse DEGs 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
MTT Assay:Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Stanley Adoro Apr 10, 2025 QuantStudio six Flex real-time PCR system for RT-qPCR and ChIP-qPCR BioRad iMark Microplate reader and SpectraMax ID3 for reading absorbance for BCA and MTT assays BD LSR Fortessa and BD LSR-II for flow cytometry acquisition Konica Minolta SRX-101a for western blot film development Analytik Jena UVP ChemStudio and BioRad ChemiDoc MP for western blot digital imaging XF96 Extracellular Flux Analyzer (Agilent Technologies) for Seahorse assays Olympus IX73 microscope for histological images Illumina HiSeq X Ten for RNA sequencing (CUTLL1) Illumina NovaSeq 6000 for RNA sequencing (murine) and ChiP sequencing Nikon Eclipse Ti2 microscope (IF images) GraphPad Prism for data analysis and statistical tests FlowJo for flow cytometry analysis ImageJ for western blot quantification and IF analysis Star aligner (v2.7.9a) for RNA sequencing alignment htseq (v0.11.4) to generate gene counts for RNAseq DEseq2 (v1.38.3) for differential expression analysis GSEA 4.3.2 and MsigDB Hallmark gene set for pathway analysis BioMart R and Ensembl release 105 for comparing human and mouse DEGs 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Flow Cytometry:Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Stanley Adoro Apr 10, 2025 QuantStudio six Flex real-time PCR system for RT-qPCR and ChIP-qPCR BioRad iMark Microplate reader and SpectraMax ID3 for reading absorbance for BCA and MTT assays BD LSR Fortessa and BD LSR-II for flow cytometry acquisition Konica Minolta SRX-101a for western blot film development Analytik Jena UVP ChemStudio and BioRad ChemiDoc MP for western blot digital imaging XF96 Extracellular Flux Analyzer (Agilent Technologies) for Seahorse assays Olympus IX73 microscope for histological images Illumina HiSeq X Ten for RNA sequencing (CUTLL1) Illumina NovaSeq 6000 for RNA sequencing (murine) and ChiP sequencing Nikon Eclipse Ti2 microscope (IF images) GraphPad Prism for data analysis and statistical tests FlowJo for flow cytometry analysis ImageJ for western blot quantification and IF analysis Star aligner (v2.7.9a) for RNA sequencing alignment htseq (v0.11.4) to generate gene counts for RNAseq DEseq2 (v1.38.3) for differential expression analysis GSEA 4.3.2 and MsigDB Hallmark gene set for pathway analysis BioMart R and Ensembl release 105 for comparing human and mouse DEGs 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Western Blot:Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Stanley Adoro Apr 10, 2025 QuantStudio six Flex real-time PCR system for RT-qPCR and ChIP-qPCR BioRad iMark Microplate reader and SpectraMax ID3 for reading absorbance for BCA and MTT assays BD LSR Fortessa and BD LSR-II for flow cytometry acquisition Konica Minolta SRX-101a for western blot film development Analytik Jena UVP ChemStudio and BioRad ChemiDoc MP for western blot digital imaging XF96 Extracellular Flux Analyzer (Agilent Technologies) for Seahorse assays Olympus IX73 microscope for histological images Illumina HiSeq X Ten for RNA sequencing (CUTLL1) Illumina NovaSeq 6000 for RNA sequencing (murine) and ChiP sequencing Nikon Eclipse Ti2 microscope (IF images) GraphPad Prism for data analysis and statistical tests FlowJo for flow cytometry analysis ImageJ for western blot quantification and IF analysis Star aligner (v2.7.9a) for RNA sequencing alignment htseq (v0.11.4) to generate gene counts for RNAseq DEseq2 (v1.38.3) for differential expression analysis GSEA 4.3.2 and MsigDB Hallmark gene set for pathway analysis BioMart R and Ensembl release 105 for comparing human and mouse DEGs 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Imaging:Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Stanley Adoro Apr 10, 2025 QuantStudio six Flex real-time PCR system for RT-qPCR and ChIP-qPCR BioRad iMark Microplate reader and SpectraMax ID3 for reading absorbance for BCA and MTT assays BD LSR Fortessa and BD LSR-II for flow cytometry acquisition Konica Minolta SRX-101a for western blot film development Analytik Jena UVP ChemStudio and BioRad ChemiDoc MP for western blot digital imaging XF96 Extracellular Flux Analyzer (Agilent Technologies) for Seahorse assays Olympus IX73 microscope for histological images Illumina HiSeq X Ten for RNA sequencing (CUTLL1) Illumina NovaSeq 6000 for RNA sequencing (murine) and ChiP sequencing Nikon Eclipse Ti2 microscope (IF images) GraphPad Prism for data analysis and statistical tests FlowJo for flow cytometry analysis ImageJ for western blot quantification and IF analysis Star aligner (v2.7.9a) for RNA sequencing alignment htseq (v0.11.4) to generate gene counts for RNAseq DEseq2 (v1.38.3) for differential expression analysis GSEA 4.3.2 and MsigDB Hallmark gene set for pathway analysis BioMart R and Ensembl release 105 for comparing human and mouse DEGs 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Microscopy:Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Stanley Adoro Apr 10, 2025 QuantStudio six Flex real-time PCR system for RT-qPCR and ChIP-qPCR BioRad iMark Microplate reader and SpectraMax ID3 for reading absorbance for BCA and MTT assays BD LSR Fortessa and BD LSR-II for flow cytometry acquisition Konica Minolta SRX-101a for western blot film development Analytik Jena UVP ChemStudio and BioRad ChemiDoc MP for western blot digital imaging XF96 Extracellular Flux Analyzer (Agilent Technologies) for Seahorse assays Olympus IX73 microscope for histological images Illumina HiSeq X Ten for RNA sequencing (CUTLL1) Illumina NovaSeq 6000 for RNA sequencing (murine) and ChiP sequencing Nikon Eclipse Ti2 microscope (IF images) GraphPad Prism for data analysis and statistical tests FlowJo for flow cytometry analysis ImageJ for western blot quantification and IF analysis Star aligner (v2.7.9a) for RNA sequencing alignment htseq (v0.11.4) to generate gene counts for RNAseq DEseq2 (v1.38.3) for differential expression analysis GSEA 4.3.2 and MsigDB Hallmark gene set for pathway analysis BioMart R and Ensembl release 105 for comparing human and mouse DEGs 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
RNA Sequencing:Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Stanley Adoro Apr 10, 2025 QuantStudio six Flex real-time PCR system for RT-qPCR and ChIP-qPCR BioRad iMark Microplate reader and SpectraMax ID3 for reading absorbance for BCA and MTT assays BD LSR Fortessa and BD LSR-II for flow cytometry acquisition Konica Minolta SRX-101a for western blot film development Analytik Jena UVP ChemStudio and BioRad ChemiDoc MP for western blot digital imaging XF96 Extracellular Flux Analyzer (Agilent Technologies) for Seahorse assays Olympus IX73 microscope for histological images Illumina HiSeq X Ten for RNA sequencing (CUTLL1) Illumina NovaSeq 6000 for RNA sequencing (murine) and ChiP sequencing Nikon Eclipse Ti2 microscope (IF images) GraphPad Prism for data analysis and statistical tests FlowJo for flow cytometry analysis ImageJ for western blot quantification and IF analysis Star aligner (v2.7.9a) for RNA sequencing alignment htseq (v0.11.4) to generate gene counts for RNAseq DEseq2 (v1.38.3) for differential expression analysis GSEA 4.3.2 and MsigDB Hallmark gene set for pathway analysis BioMart R and Ensembl release 105 for comparing human and mouse DEGs 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
ChIP-sequencing:Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Stanley Adoro Apr 10, 2025 QuantStudio six Flex real-time PCR system for RT-qPCR and ChIP-qPCR BioRad iMark Microplate reader and SpectraMax ID3 for reading absorbance for BCA and MTT assays BD LSR Fortessa and BD LSR-II for flow cytometry acquisition Konica Minolta SRX-101a for western blot film development Analytik Jena UVP ChemStudio and BioRad ChemiDoc MP for western blot digital imaging XF96 Extracellular Flux Analyzer (Agilent Technologies) for Seahorse assays Olympus IX73 microscope for histological images Illumina HiSeq X Ten for RNA sequencing (CUTLL1) Illumina NovaSeq 6000 for RNA sequencing (murine) and ChiP sequencing Nikon Eclipse Ti2 microscope (IF images) GraphPad Prism for data analysis and statistical tests FlowJo for flow cytometry analysis ImageJ for western blot quantification and IF analysis Star aligner (v2.7.9a) for RNA sequencing alignment htseq (v0.11.4) to generate gene counts for RNAseq DEseq2 (v1.38.3) for differential expression analysis GSEA 4.3.2 and MsigDB Hallmark gene set for pathway analysis BioMart R and Ensembl release 105 for comparing human and mouse DEGs 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
RNA sequencing:Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Stanley Adoro Apr 10, 2025 QuantStudio six Flex real-time PCR system for RT-qPCR and ChIP-qPCR BioRad iMark Microplate reader and SpectraMax ID3 for reading absorbance for BCA and MTT assays BD LSR Fortessa and BD LSR-II for flow cytometry acquisition Konica Minolta SRX-101a for western blot film development Analytik Jena UVP ChemStudio and BioRad ChemiDoc MP for western blot digital imaging XF96 Extracellular Flux Analyzer (Agilent Technologies) for Seahorse assays Olympus IX73 microscope for histological images Illumina HiSeq X Ten for RNA sequencing (CUTLL1) Illumina NovaSeq 6000 for RNA sequencing (murine) and ChiP sequencing Nikon Eclipse Ti2 microscope (IF images) GraphPad Prism for data analysis and statistical tests FlowJo for flow cytometry analysis ImageJ for western blot quantification and IF analysis Star aligner (v2.7.9a) for RNA sequencing alignment htseq (v0.11.4) to generate gene counts for RNAseq DEseq2 (v1.38.3) for differential expression analysis GSEA 4.3.2 and MsigDB Hallmark gene set for pathway analysis BioMart R and Ensembl release 105 for comparing human and mouse DEGs 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Quantitative Proteomics:Article Title: The ESCRT protein CHMP5 promotes T cell leukemia by enabling BRD4-p300-dependent transcription
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Data analysis Stanley Adoro Apr 10, 2025 QuantStudio six Flex real-time PCR system for RT-qPCR and ChIP-qPCR BioRad iMark Microplate reader and SpectraMax ID3 for reading absorbance for BCA and MTT assays BD LSR Fortessa and BD LSR-II for flow cytometry acquisition Konica Minolta SRX-101a for western blot film development Analytik Jena UVP ChemStudio and BioRad ChemiDoc MP for western blot digital imaging XF96 Extracellular Flux Analyzer (Agilent Technologies) for Seahorse assays Olympus IX73 microscope for histological images Illumina HiSeq X Ten for RNA sequencing (CUTLL1) Illumina NovaSeq 6000 for RNA sequencing (murine) and ChiP sequencing Nikon Eclipse Ti2 microscope (IF images) GraphPad Prism for data analysis and statistical tests FlowJo for flow cytometry analysis ImageJ for western blot quantification and IF analysis Star aligner (v2.7.9a) for RNA sequencing alignment htseq (v0.11.4) to generate gene counts for RNAseq DEseq2 (v1.38.3) for differential expression analysis GSEA 4.3.2 and MsigDB Hallmark gene set for pathway analysis BioMart R and Ensembl release 105 for comparing human and mouse DEGs 2 n atu re p o rtfo lio | rep o rtin g su m m ary A pril2023 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers. ..
Cytometry:Article Title: Taurine from tumour niche drives glycolysis to promote leukaemogenesis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Flow cytometry-based analysis was performed on LSR Fortessa and cell sorting was carried out on BD FACS Aria II using FACSDiva v9.0 (BD Biosciences). qRT-PCR was carried out on BioRad CFX96 C100 Thermocycler using BioRad CFX Manager 1.1 v4.1 (BioRad) and Thermofisher Scientific Quant Studio 12K Flex Real Time PCR using using Quant Studio v1.2 (Thermofisher Scientific). .. RNA and DNA concentrations were determined using the NanoDrop 1000 Spectrophotometer (Thermofisher Scientific).
Article Title: Taurine from tumour niche drives glycolysis to promote leukaemogenesis.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Flow cytometry-based analysis was performed on LSR Fortessa and cell sorting was carried out on BD FACS Aria II using FACSDiva v9.0 (BD Biosciences). qRT-PCR was carried out on BioRad CFX96 C100 Thermocycler using BioRad CFX Manager 1.1 v4.1 (BioRad) and Thermofisher Scientific Quant Studio 12K Flex Real Time PCR using using Quant Studio v1.2 (Thermofisher Scientific). .. RNA and DNA concentrations were determined using the NanoDrop 1000 Spectrophotometer (Thermofisher Scientific).
FACS:Article Title: Taurine from tumour niche drives glycolysis to promote leukaemogenesis
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Flow cytometry-based analysis was performed on LSR Fortessa and cell sorting was carried out on BD FACS Aria II using FACSDiva v9.0 (BD Biosciences). qRT-PCR was carried out on BioRad CFX96 C100 Thermocycler using BioRad CFX Manager 1.1 v4.1 (BioRad) and Thermofisher Scientific Quant Studio 12K Flex Real Time PCR using using Quant Studio v1.2 (Thermofisher Scientific). .. RNA and DNA concentrations were determined using the NanoDrop 1000 Spectrophotometer (Thermofisher Scientific).
Article Title: Taurine from tumour niche drives glycolysis to promote leukaemogenesis.
Article Snippet: .. Software and code Policy information about availability of computer code Data collection Flow cytometry-based analysis was performed on LSR Fortessa and cell sorting was carried out on BD FACS Aria II using FACSDiva v9.0 (BD Biosciences). qRT-PCR was carried out on BioRad CFX96 C100 Thermocycler using BioRad CFX Manager 1.1 v4.1 (BioRad) and Thermofisher Scientific Quant Studio 12K Flex Real Time PCR using using Quant Studio v1.2 (Thermofisher Scientific). .. RNA and DNA concentrations were determined using the NanoDrop 1000 Spectrophotometer (Thermofisher Scientific).
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